cd10 pe cy7  (Cytek Biosciences)

Journal: STAR Protocols

Article Title: Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood

doi: 10.1016/j.xpro.2024.103024

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Article Snippet: Anti-human CD10-PE-Cy7 (clone HI10a; 2:50) , BD Biosciences , Cat#565282.

Techniques: Blocking Assay, Recombinant, Lysis, Software

Journal: STAR Protocols

Article Title: Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood

doi: 10.1016/j.xpro.2024.103024

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Article Snippet: Anti-human CD10-PE-Cy7 (clone HI10a; 2:50) , BD Biosciences , Cat#565282.

Techniques: Marker

Journal: STAR Protocols

Article Title: Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood

doi: 10.1016/j.xpro.2024.103024

Figure Lengend Snippet:

Article Snippet: Anti-human CD10-PE-Cy7 (clone HI10a; 2:50) , BD Biosciences , Cat#565282.

Techniques:

Journal: Cell Reports Medicine

Article Title: Insights into highly engraftable hematopoietic cells from 27-year cryopreserved umbilical cord blood

doi: 10.1016/j.xcrm.2023.101259

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Article Snippet: Anti-Human CD10-PE-Cy7 (Clone HI10a) , BD Biosciences , Cat#565282; AB_2739153.

Techniques: Recombinant, In Vivo, Software

Journal: BMC Immunology

Article Title: Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis

doi: 10.1186/1471-2172-7-13

Figure Lengend Snippet: Wnt3A induces β-catenin stabilization in BM B progenitor cells . Western blot analysis of β-catenin levels in BM CD10 + B lineage progenitor cells stimulated with Wnt3A (100 ng/ml) or vehicle (PBS with 0.1% detoxified BSA) for 3 hours. The blots were incubated with an Ab against β-catenin, followed by an Ab against β-actin to ascertain equal loading in the wells. The same results were found in cells from 4 out of 5 different donors, indicating some degree of donor variation in the response to Wnt3A.

Article Snippet: The following monoclonal antibodies (mAbs) were used for flow cytometry: anti-CD34 PE, anti-CD10 APC, anti-CD10 PE-Cy7 and anti-CD20 APC from Becton Dickinson, Biosciences Pharmingen (San Jose, CA, USA), anti-CD19 PE-Cy5 and anti-CD34 PE-Cy5 from Immunotech (Marseille, France) and anti-CD19 PE, anti-CD45 PE and anti-IgM Fitc from Dako Cytomation (Denmark).

Techniques: Western Blot, Incubation

Journal: BMC Immunology

Article Title: Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis

doi: 10.1186/1471-2172-7-13

Figure Lengend Snippet: Wnt3A inhibits in vitro B lymphopoiesis . BM CD133 + CD10 - HPC (A: assay 1) or CD10 + B progenitor cells (B: assay 2) were cocultured with a confluent layer of the murine stromal cell line MS-5 for 3 or 2 weeks, respectively, while treated with Wnt3A (100 ng/ml), Wnt3A + sFRP1 (2 μg/ml), Wnt3A + Dkk1 (500 ng/ml) or medium only. The number of resulting CD19 + B lineage cells in each sample was determined by quantitative flow cytometry. The percentage of CD34 + cells among the CD19 + cells were measured before and after culturing, with and without treatment with Wnt3A (C). The bars represent the mean of N experiments performed in duplicate, ± SEM. A) N = 6. B) Cells treated with control medium or Wnt3A: N = 11, Wnt3A + sFRP1: N = 3, Wnt3A + Dkk1: N = 2. C) day 0: N = 7, day 7: N = 3, Day 14: N = 8. *p ≤ 0.01, Wilcoxon Signed Ranks Test.

Article Snippet: The following monoclonal antibodies (mAbs) were used for flow cytometry: anti-CD34 PE, anti-CD10 APC, anti-CD10 PE-Cy7 and anti-CD20 APC from Becton Dickinson, Biosciences Pharmingen (San Jose, CA, USA), anti-CD19 PE-Cy5 and anti-CD34 PE-Cy5 from Immunotech (Marseille, France) and anti-CD19 PE, anti-CD45 PE and anti-IgM Fitc from Dako Cytomation (Denmark).

Techniques: In Vitro, Flow Cytometry

Journal: BMC Immunology

Article Title: Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis

doi: 10.1186/1471-2172-7-13

Figure Lengend Snippet: Number of CD19 cells after two weeks of culturing BM CD10 + cells on stromal cells

Article Snippet: The following monoclonal antibodies (mAbs) were used for flow cytometry: anti-CD34 PE, anti-CD10 APC, anti-CD10 PE-Cy7 and anti-CD20 APC from Becton Dickinson, Biosciences Pharmingen (San Jose, CA, USA), anti-CD19 PE-Cy5 and anti-CD34 PE-Cy5 from Immunotech (Marseille, France) and anti-CD19 PE, anti-CD45 PE and anti-IgM Fitc from Dako Cytomation (Denmark).

Techniques: Inhibition

Journal: BMC Immunology

Article Title: Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis

doi: 10.1186/1471-2172-7-13

Figure Lengend Snippet: Wnt3A inhibits the initial phase of stromal supported cell division of BM B progenitors . Highly purified BM CD10 + CFSE mean cells were grown on a confluent layer of MS-5 and treated with Wnt3A (25–400 ng/ml) or medium only. After three days, the cells were analyzed on a FACScan flow cytometer for the number of cell divisions of CD19 + cells. A) Tracking histograms of cell divisions of CFSE-labeled BM B progenitor cells in the presence or absence of Wnt3A (100 ng/ml) One representative experiment of six is shown. B) Dose dependent inhibition of cell division of CD34 + pro-B cells and CD34 - pre-B cells by Wnt3A (closed circles). The inhibitory effect of Wnt3A was blocked by Wnt antagonist sFRP1 (2 μg/ml) (open circle). Data are shown as percentage of cells that had gone through one or more cell divisions, as determined by cell division tracking with CFSE. One representative experiment of two is shown, except for Wnt3A (100 ng/ml) and Wnt3A + FRP1 (2 μg/ml) where one representative experiment of six is shown (*p < 0.05, Wilcoxon Signed Ranks Test, n = 6).

Article Snippet: The following monoclonal antibodies (mAbs) were used for flow cytometry: anti-CD34 PE, anti-CD10 APC, anti-CD10 PE-Cy7 and anti-CD20 APC from Becton Dickinson, Biosciences Pharmingen (San Jose, CA, USA), anti-CD19 PE-Cy5 and anti-CD34 PE-Cy5 from Immunotech (Marseille, France) and anti-CD19 PE, anti-CD45 PE and anti-IgM Fitc from Dako Cytomation (Denmark).

Techniques: Purification, Flow Cytometry, Labeling, Inhibition